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These harmful molecules, known as free radicals, when removed, significantly enhance the immune system

In addition, glucose enters the brain from the blood by crossing the bloodbrain barrier through glucose transporter 1 (GLUT1)

Thus, we could not quantitatively compare the production of ROS in the two cell lines, we could only conclude that both cell lines produce ROS in response to rotenone

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its standard practice in any serious lab for a few good reasons: It Preserves the Master Stock: You only ever thaw the exact amount you need for a single experiment